Genotyping method for point mutation detection in the intestinal fatty acid binding protein, using fluorescent probes.
نویسندگان
چکیده
The difference between serum and plasma potassium concentrations is well known, but we believe the clinical significance of the intra-and interindividual variability of the difference is not fully appreciated. This variability differs between types of blood collection tubes, possibly because of the extent of clot retraction induced by the surface of the tube or the clotting accelerator. Even for the sample tube showing the least variable potassium release (tube D; Becton Dickinson), serum remains an unsuitable medium for managing patients in whom potassium ho-meostasis is important, particularly because most standard medical texts base management on plasma concentrations (5–7). The extent of potassium release during clotting or during delays in the separation of serum from cells means that a true plasma potassium in the middle of the reference interval (4.16 mmol/L) may range from 3.82 to 5.44 mmol/L in a simultaneously taken serum sample (tube C); the variation is unpredictable, unrelated to the plasma concentration, and additive to the inherent biological variation in potassium concentrations. A reference interval derived from serum samples would be wider than the interval derived from plasma and would cause truly abnormal concentrations to be masked, e.g., hyper-kalemia associated with a small release on clotting or hypokalemia associated with a large release on clotting. These studies were conducted on hospital outpatients, but because we instituted a policy of requesting plasma when a serum result fell outside the reference interval, we have observed even greater differences in hospital inpa-tients with disorders, such as diabetic ketoacidosis, in which the potassium balance may be critical. Plasma is the preferred medium for the determination of potassium concentration, although it is not ideal for many other biochemical analyses. When serum is to be used, laboratories should be aware of the additional variability observed with this medium and the extent to which this is affected by their choice of sample tube. A comparison of serum versus heparinized plasma for routine chemical tests. A comparison of serum electrolyte concentrations in blood collected by evacuated tubes or syringes. The intestinal fatty acid-binding protein (IFABP) is located in the intestine and is involved with long-chain fatty acid transport and metabolism (1). The FABP2 gene at chromosome 4q28-31 encodes IFABP. Genetic variation at this locus could lead to altered fatty acid absorption and energy metabolism. A common point mutation (G3 A) in the gene for IFABP, with an allele frequency of the A allele of ϳ0.29 (2), …
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عنوان ژورنال:
- Clinical chemistry
دوره 45 7 شماره
صفحات -
تاریخ انتشار 1999